rabbit polyclonal anti ph3  (Cell Signaling Technology Inc)

Journal: Kidney International Reports

Article Title: Prostaglandin Analogs and Eupatilin as Treatments for Nephronophthisis

doi: 10.1016/j.ekir.2025.04.060

Figure Lengend Snippet: Figure 6. PGE2, but not Eupatilin, shows beneficial effect on cysts size in traf3ip1 mutant fish. (a) Representative pictures obtained after crossing m649-GFP fish (48 hpf) with an example of straight (up) and curved (down) embryos. The curvature phenotype was quantified (n ¼ 8 experiments). (b) Representative pictures of the GFP-expressing pronephros with, from left to right: wild-type, mild (unilateral or small), or severe (bilateral) cystic pronephros. The region of the pronephros used to quantify the cyst area is indicated by a dotted white line. (c) Quantification of cyst severity score in straight and curved embryos (n ¼ 4 experiments, chi-square test: ****P < 0.0001). (d) Quantification of cyst area measured as explained in (b) for straight and curved embryos (n ¼ 4 experiments; unpaired t test with Welch’s correction: ****P < 0.0001; error bars representing mean SD. (e) Straight (upper panels) and curved (lower panels) m649-GFP embryos were fixed and stained for cilia (acetylated-atubulin, AcTub; red) and mitotic cells (phospho-histone-H3, PH3; purple). Representative color images are shown on the left. The areas surrounded by dotted lines represent one side of the glomerulus with the initial part of the proximal tubule based on GFP staining. On the right, zoomed (2.33) views of the same samples are shown for AcTub only. White arrowheads on the left and arrows on the right pictures indicate mitotic cells and cilia, respectively. Scale bars: 20mm. (f) Quantification of cilia number per glomerulus for straight and curved embryos. Unpaired t test with Welch’s correction: **** P < 0.0001. (g) Quantification of mitotic cells number per glomerular region for straight and curved embryos. Mann-Whitney test: *P < 0.05. (h and i) m649-GFP embryos were treated with DMSO, dimethyl-PGE2 (dmPGE2; 100 mM), or Eupatilin (2.5 mM) starting at 24 hpf. Quantification of the effects of treatments on cyst area (dmPGE2: Mann-Whitney test; ****P < 0.0001; Eupatilin: unpaired t test with Welch’s correction: ns P > 0.05). DMSO, dimethyl sulfoxide; EUP, Eupatilin.

Article Snippet: Antibodies The following antibodies were used: acetyl-a tubulin mouse monoclonal (1/300; Sigma, T6793), ADCY3 rabbit polyclonal (1/100e; Invitrogen, PA5-35382), ARL13B rabbit polyclonal (1/800e; ProteinTech, 17711-1-AP), ARL13B mouse monoclonal (1/100e; Kidney International Reports (2025) -, -–- FLA 5.7.0 DTD EKIR3376_proof 16 ABCAM, ab136648), INPP5E rabbit polyclonal (1/200e; Proteintech, 17797-1-AP), g-Tubulin mouse monoclonal (1/5000e; Sigma, T6557), GT335 mouse monoclonal (1/5000e; Adipogen, AG-20B-0020-C100), PH3 rabbit polyclonal (1/200; Cell Signaling Technology, 3377S), NPHP4 rabbit polyclonal (1/100e; BiCell scientific, 90004), and NPHP11 rabbit polyclonal (1/100e; BiCell, 90103).

Techniques: Mutagenesis, Expressing, Staining, MANN-WHITNEY

Journal: eLife

Article Title: Delta-dependent Notch activation closes the early neuroblast temporal program to promote lineage progression and neurogenesis termination in Drosophila

doi: 10.7554/eLife.88565

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-PH3 (rabbit polyclonal) , Millipore , 06-570 , IF (1:1000).

Techniques: Electron Microscopy, Software

Journal: iScience

Article Title: KMT2D suppresses Sonic hedgehog-driven medulloblastoma progression and metastasis

doi: 10.1016/j.isci.2023.107831

Figure Lengend Snippet:

Article Snippet: Rabbit Polyclonal anti-PH3 , EMD Millipore , 06–570.

Techniques: Purification, Imaging, EdU Assay, Staining, Recombinant, SYBR Green Assay, Magnetic Beads, Western Blot, cDNA Synthesis, In Situ Hybridization, Software